Immune Response to Inactivated Newcastle Disease Virus by Electrolysed Catholyte Anolyte and Binary Ethylenimine in Specific Pathogen Free Chickens

This study aimed to investigate the immunogenicity of Newcastle disease virus (NDV) in specific-pathogen-free (SPF) chickens. The NDV was inactivated using either Binary ethyleneimine (BEI) or Electrolysed water-Catholyte-Anolyte (ECA). Complete inactivation of NDV occurred after 24 hours with either BEI or ECA. Prepared inactivated NDV vaccines were tested for their efficiency in generating humoral immune response in different groups of specific pathogen free (SPF) chicks. Test groups received 0.2 ml BEI inactivated NDV (NDVBEI) and ECA (NDVECA) subcutaneously. No significant ELISA total mean titer between NDVBEI group (11303 ± 4515) and NDVECA (12131 ± 1932) (p < 0.05) at two week post inoculation. BEI and ECA inactivated vaccine gave higher antibody titers and preserves both structural integrity and antigenicity of the virus. Thus, it might be possible to use these compounds as an inactivator agent for commercial NDV inactivated vaccines in future.