Author/Authors :
Mohammed، M H نويسنده Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Mohammed, M H , Hair-Bejo، M نويسنده Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Hair-Bejo, M , Omar، A R نويسنده Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Omar, A R , Aini، I نويسنده Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Aini, I , Hasoon، Mauida F نويسنده Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia Hasoon, Mauida F
Abstract :
This study aimed to investigate the efficacy, safety and immunogenicity of the attenuated and inactivated Malaysian isolate of vvIBDV (UPM0081) in specific-pathogen-free (SPF) chickens. The UPM0081T15 passage 15 and UPM0081T20 passage 20 of vvIBDV attenuated in Vero cells were inactivated using water-Catholyte-Anolyte (ECA). Complete inactivation of UPM0081T15 with titer of 106.7 TCID50/0.1 mL and UPM0081T20 with titer of 107.4 TCID50/0.1mL occurred after 24 hours. The inactivated virus suspension and an equal volume of Freund’s incomplete adjuvant were mixed together water-in-oil emulsion and injected subcutaneously into 42-day-old SPF chickens. Neither clinical signs nor gross lesions were observed in chickens before and after vvIBDV challenged. High and protective level of IBD antibody titer was recorded into 2 groups at 2 weeks post infection and 2 weeks post challenged. The study showed that both the inactivated UPM0081T15 and UPM0081T20 was safe and could provide 100% protection against vvIBDV challenged with titer of 107.8 EID50/ 0.1 mL.
