Author/Authors :
Eslami، g نويسنده Department of Parasitology and Mycology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Zohourtabar، a نويسنده Department of Food Hygiene and Safety, Faculty of Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Mehrizi، s r نويسنده Official Meat Inspector at Yazd Industrial Slaughterhouse, Yazd, Iran ,
Abstract :
Introduction: The main agents of sarcocystosis in cattle as an intermediate host include S. cruzi, S.
hominis and S. hirsuta. A sensitive and specific tool such as molecular-based techniques would be
necessary to identify the species.
Case report: After collection of beef sample from Yazd slaughterhouse, DNA extraction was done
with salting out method. The 18SrRNA gene as a specific target gene was used for molecular
detection of Sarcocystis spp, then Restriction Fragment Length Polymorphysm (RFLP) analysis
identified the speciesusing Rsa and Bfa. Results showed that our designed molecular method could
identify S. hirsuta in beef sample.
Conclusion: Based on our knowledge, this study indicates the first report of molecular
identification of S. hirsuta in Iran.
