Author/Authors :
Pooladi، Mehdi نويسنده Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Rezaei-Tavirani، Mostafa نويسنده , , Hashemi، Mehrdad نويسنده , , Hesami -Tackallou ، Saeed نويسنده Biology of Department , , Khaghani-Razi-Abad، Solmaz نويسنده Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Moradi، Afshin نويسنده Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , , Zali، Alireza نويسنده , , Mousavi، Masoumea نويسنده Proteomics Research Center, Faculty of Paramedical Sciences , , Rakhshan، Azadeh نويسنده , , Firozi-Dalvand، Leila نويسنده Dept. of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran , , Omidi، Roghaye نويسنده Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,
Abstract :
Background: Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding.
Methods: The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks.
Results: The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values.
Conclusion: This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity.
