Author/Authors :
Saffarzadeh، Negin نويسنده Department of Medical Genetic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Kalantar، Seyed Mehdi نويسنده , , Jebali، Ali نويسنده Department of Medical Nanotechnology, Pajoohesh Lab, Yazd, Iran. , , Hekmatimoghaddam، Seyed Hossein نويسنده Department of Laboratory Sciences, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, , , Sheikhha، Mohammad Hassan نويسنده , , Ghasemi، Nasrin نويسنده , , Farashahi، Ehsan نويسنده Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran ,
Abstract :
Objective(s): Although several chemical and physical methods for gene delivery have been
introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability
remain the main issues. In this study, hydroxyapatite nanoparticles (NPs) and 1,2-dioleoyl-snglycero-
3-phosphoethanolamine (DOPE)-modified hydroxyapatite NPs was coated with
antisense oligonucleotide of E6 mRNA, and their uptakes into the cervical cancer cell line
were evaluated.
Materials and Methods: Calcium nitrate and diammonium phosphate were used for the
synthesis of the hydroxyapatite nanoparticle. Thus, they were coated with polyethylene glycol
(PEG), DOPE and antisense oligonucleotide of E6 mRNA using a cross-linker. Then,
hydroxyapatite NPs and DOPE-modified hydroxyapatite NPs were incubated 48 hours with
cervical cancer cells and their uptakes were evaluated by fluorescent microscopy.
Results: The hydroxyapatite NPs had different shapes and some agglomeration with average
size of 100 nm. The results showed DOPE-modified hydroxyapatite NPs had higher uptake
than hydroxyapatite NPs (P < 0.05).
Conclusions: Hydroxyapatite NPs conjugated with DOPE are a good choice for gene delivery
and silencing of viral genes in cervical cancer cells, but their efficacy should be addressed
more in future studies.
