Title of article :
Diagnosis of canine visceral leishmaniasis: Biotechnological advances
Author/Authors :
Gomes، نويسنده , , Y.M. and Paiva Cavalcanti، نويسنده , , M. Meléndez-Lira، نويسنده , , R.A. and Abath، نويسنده , , F.G.C. and Alves، نويسنده , , L.C.، نويسنده ,
Issue Information :
فصلنامه با شماره پیاپی سال 2008
Pages :
8
From page :
45
To page :
52
Abstract :
Human visceral leishmaniasis (HVL) is endemic in the tropical and sub-tropical regions of Africa, Asia, the Mediterranean, Southern Europe and South and Central America, with approximately 500,000 new cases reported annually. As dogs are considered to be the major reservoirs for HVL, the accurate diagnosis of disease in these animals is important. Diagnosis of canine visceral leishmaniasis (CVL) is performed mainly by direct parasitological methods that can yield false-negative results, either because of the very low number of Leishmania spp. organisms in clinical samples (bone marrow and lymph nodes) or because morphological identification is difficult. In addition, these methods are invasive. Conventional serological techniques are limited by cross-reactivity with other parasitic diseases and because several technical procedures have not been standardised. The development of polymerase chain reaction based approaches and immunoassays based on the use of recombinant antigens aimed at improving the sensitivity and specificity of CVL diagnosis is discussed.
Keywords :
Canine visceral leishmaniasis , Parasitological methods , Immunological methods , Molecular approaches
Journal title :
The Veterinary Journal
Serial Year :
2008
Journal title :
The Veterinary Journal
Record number :
1392187
Link To Document :
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