Author/Authors :
Ramيrez، نويسنده , , Ana S. and Fleitas، نويسنده , , Juan Luis and Rosales، نويسنده , , Rubén S. and Poveda، نويسنده , , Carlos and de la Fe، نويسنده , , Christian and Andrada، نويسنده , , Marisa and Castro، نويسنده , , Ayoze and Poveda، نويسنده , , José B.، نويسنده ,
Abstract :
The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri.
Keywords :
Mollicutes , Animal sera , Mycoplasma species , culture media
