Author/Authors :
Webb، نويسنده , , Katy and Barker، نويسنده , , Colin and Harrison، نويسنده , , Tihana and Heather، نويسنده , , Zoe and Steward، نويسنده , , Karen F. and Robinson، نويسنده , , Carl and Newton، نويسنده , , J. Richard and Waller، نويسنده , , Andrew S.، نويسنده ,
Abstract :
Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.
Keywords :
equine , Strangles , Streptococcus equi , Triplex quantitative PCR
