Author/Authors :
Silva، نويسنده , , Maria Angelica and Altamura، نويسنده , , Gennaro and Corteggio، نويسنده , , Annunziata and Roperto، نويسنده , , Franco and Bocaneti، نويسنده , , Florentina and Velescu، نويسنده , , Elena and Freitas، نويسنده , , Antonio C. C. Carvalho، نويسنده , , Cybelle C.R. and Cavalcanti، نويسنده , , Karen P.S. and Borzacchiello، نويسنده , , Giuseppe، نويسنده ,
Abstract :
Bovine papillomaviruses (BPVs) can infect epithelial cells and fibroblasts, inducing fibropapillomas in cattle. Gap junctions are communication channels between cells composed of connexins (Cxs). This study evaluated expression of Cx26 and the major BPV oncoprotein E5 in bovine cutaneous fibropapillomas. BPV DNA was amplified from 20/20 fibropapillomas and 3/3 samples of normal skin. All fibropapillomas (20/20) were positive by immunostaining for E5, whereas the three normal skin samples were negative. Cx26 was expressed faintly in the normal skin epithelium. Positive cytoplasmic and juxtanuclear immunoreactivity for Cx26 was evident in 18/20 (90%) fibropapillomas. Western blot analysis demonstrated higher expression of Cx26 in 6/6 fibropapillomas compared to normal skin samples.
Keywords :
Bovine papillomavirus , E5 , connexin , Bovine cutaneous fibropapillomas
