Author/Authors :
Li، نويسنده , , Hua-tao and Sun، نويسنده , , Ling-suang and Chen، نويسنده , , Zhong-ming and Hu، نويسنده , , Jing-si and Ye، نويسنده , , Cun-dong and Jia، نويسنده , , Kun and Wang، نويسنده , , Heng and Yuan، نويسنده , , Li-guo and Zhang، نويسنده , , Gui-hong and Li، نويسنده , , Shoujun، نويسنده ,
Abstract :
Anaplasma platys is a parasite of canine platelets that causes infectious cyclic thrombocytopenia. In this study, a novel real-time loop-mediated isothermal amplification (RT-LAMP) method was developed to detect A. platys. RT-LAMP primer sets were designed using a citrate synthase gene sequence and the assay was performed at 63 °C for 30 min. No cross-reactivity was observed with other Anaplasma or Ehrlichia spp. and the method exhibited a similar level of sensitivity in detecting the organism in 58 canine blood samples to that of a nested PCR. This RT-LAMP is a rapid and potentially cost-effective method of diagnosing A. platys infection in dogs.
Keywords :
canine , Anaplasma platys , gltA gene , Real-time loop-mediated isothermal amplification
