Amperometric enzyme electrode for l(+)-lactate determination using immobilized l(+)-lactate oxidase in poly(vinylferrocenium) film

The steady-state amperometric measurements of l(+)-lactate was performed by an enzyme electrode which was developed by using immobilization of the l(+)-lactate oxidase (LOx) in poly(vinylferrocenium) (PVF+), film coated on a Pt electrode. The effects of temperature, pH, polymer film thickness and enzyme concentration were investigated. The linear concentration range was found to be between 0.05 and 0.60 mM for this enzyme electrode. The maximum current value obtained was higher than those of other studies. The enzyme electrode was found to be stable for about 15 days using the current values measured daily for the same substrate concentration.