Author/Authors :
E. KOUBOVA، نويسنده , , V. and Brynda، نويسنده , , E. and Karasov?، نويسنده , , L. and ?kvor، نويسنده , , J. and Homola، نويسنده , , J. and Dost?lek، نويسنده , , J. and Tobi?ka، نويسنده , , P. and Ro?ick?، نويسنده , , J.، نويسنده ,
Abstract :
Salmonella enteritidis and Listeria monocytogenes were detected in real time using a surface plasmon resonance (SPR) sensor based on prism excitation of surface plasmons and spectral interrogation. The respective antibodies against the pathogens were immobilized on the gold sensor surface as a covalently crosslinked double-layer or covalently bound on a crosslinked albumin layer. Salmonella and Listeria could be detected by the sensor at concentrations down to 106 cell/ml. The sensor sensitivity was comparable with that of standard ELISA in which the same antibodies were used.
