Author/Authors :
Varastehmoradi، Bardia نويسنده Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research center, Tehran University of Medical Sciences, Tehran, Iran , , Davoodi، Sayyed Mohammad Reza نويسنده PhD Student, Faculty of Management, Islamic Azad University, Dehaghan Branch, Isfahan, Iran , , Balu، Eswarapriya نويسنده Department of Biotechnology, St. Michael College of Engineering and Technology, Kalayarkoil, India , , Kalirajan، Jegatheesan نويسنده Department of Biotechnology, St. Michael College of Engineering and Technology, Kalayarkoil, India , , Zare، Bijan نويسنده Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran , , SHAHVERDI، AHMAD-REZA نويسنده ,
Abstract :
Objective(s): Electromagnetic radiations which have lethal effects on the living cells are
currently also considered as a disinfective physical agent.
Materials and Methods: In this investigation, silver nanoparticles were applied to enhance
the lethal action of low powers (100 and 180 W) of 2450 MHZ electromagnetic radiation
especially against Escherichia coli ATCC 8739. Silver nanoparticles were biologically
prepared and used for next experiments. Sterile normal saline solution was prepared and
supplemented by silver nanoparticles to reach the sub-inhibitory concentration (6.25?g/mL).
Such diluted silver colloid as well as free-silver nanoparticles solution was inoculated along
with test microorganisms, particularly E. coli. These suspensions were separately treated by
2450 MHz electromagnetic radiation for different time intervals in a microwave oven
operated at low powers (100 W and 180 W). The viable counts of bacteria before and after
each radiation time were determined by colony-forming unit (CFU) method.
Results: Results showed that the addition of silver nanoparticles significantly decreased the
required radiation time to kill vegetative forms of microorganisms. However, these
nanoparticles had no combined effect with low power electromagnetic radiation when used
against Bacillus subtilis spores.
Conclusion: The cumulative effect of silver nanoparticles and low powers electromagnetic
radiation may be useful in medical centers to reduce contamination in polluted derange and
liquid wastes materials and some devices
Abstract :
Objective(s): Electromagnetic radiations which have lethal effects on the living cells are currently also considered as a disinfective physical agent.
Materials and Methods: In this investigation, silver nanoparticles were applied to enhance the lethal action of low powers (100 and 180 W) of 2450 MHZ electromagnetic radiation especially against Escherichia coli ATCC 8739. Silver nanoparticles were biologically prepared and used for next experiments. Sterile normal saline solution was prepared and supplemented by silver nanoparticles to reach the sub-inhibitory concentration (6.25 µg/mL). Such diluted silver colloid as well as free-silver nanoparticles solution was inoculated along with test microorganisms, particularly E. coli. These suspensions were separately treated by 2450 MHz electromagnetic radiation for different time intervals in a microwave oven operated at low powers (100 W and 180 W). The viable counts of bacteria before and after each radiation time were determined by colony-forming unit (CFU) method.
Results: Results showed that the addition of silver nanoparticles significantly decreased the required radiation time to kill vegetative forms of microorganisms. However, these nanoparticles had no combined effect with low power electromagnetic radiation when used against Bacillus subtilis spores.
Conclusion:The cumulative effect of silver nanoparticles and low powers electromagnetic radiation may be useful in medical centers to reduce contamination in polluted derange and liquid wastes materials and some devices.
