Author/Authors :
Poorsattar Bejeh-Mir، Arash Arash نويسنده Dental Materials Research Center, Dentistry School, Babol University of Medical Sciences, Babol, Iran. Poorsattar Bejeh-Mir, Arash Arash , Parsian، Hadi نويسنده Department of Biochemistry & Biophysics, Babol University of Medical Sciences, Babol, Iran. , , Akbari Khoram، Maryam Maryam نويسنده Private Practice, Mazandaran Province, Iran. Akbari Khoram, Maryam Maryam , Ghasemi، Nafiseh نويسنده Dental Student, Faculty of Dentistry, Babol University of Medical Sciences , , Bijani ، Ali نويسنده Non-Communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran , , Khosravi Samani ، Mahmoud نويسنده Department of Periodontics, Dental School, Babol University of Medical Sciences, Babol, Iran. ,
Abstract :
Diagnosis of subclinical and early stage clinical periodontal dysfunction could prevent from further socioeconomic burden. The aim of this study was to assess the diagnostic applicability of nitric oxide and its end-metabolites in periodontal tissue health and disease. Forty-two patients were enrolled and divided into three groups according to gingivitis (GI) and clinical attachment level (CAL) indices: a healthy group (GI < 1, CAL < 1), b: gingivitis (GI > 1, CAL > 1) and c: periodontitis (CAL > 1) with 14 patients in each group. Unstimulated saliva and gingival crevicular fluid (GCF) were collected. Samples were evaluated for nitrite, nitrate and total nitric oxide contents with the ELISA method. In addition, CAL, GI, plaque index (PI), decay, missing, filling (DMFT) and bleeding index (BI) scores were also recorded. Except for GCF nitrite content (P= 0.89), there was an increasing trend for measured biomarkers in both saliva and GCF (Periodontitis > gingivitis > healthy periodontium, P < 0.05). Data remained stable after simultaneous adjustment for DMFT and BI scores as confounding factors. Sensitivity, specificity, positive predictive value, negative predictive value, cut point and p- value were as the followings: GCF nitrate (0.71, 0.11, 0.29,0.43, 4.97, P= 0.04), nitric oxide GCF ( 0.64, 0.18, 0.28, 0.5, 10.12, P= 0.04), nitrite saliva (0.93, 0.96,0.93,0.96,123.48, P < 0.001), salivary nitrate (0.93, 0.96, 0.93, 0.96, 123.6, P < 0.001), salivary nitric oxide (0.93, 0.96, 0.93, 0.96, 246.65, P < 0.001). Our results revealed that NO plays an important role in the process of destruction of periodontal tissues. Within the limitation of our study, detecting NO biomarker and its end metabolites in saliva is of more value to assess the periodontal health comparing to GCF.
