Title of article :
Fluorescence competition assay for the assessment of green leaf volatiles and trans-β-farnesene bound to three odorant-binding proteins in the wheat aphid Sitobion avenae (Fabricius)
Author/Authors :
Zhong، نويسنده , , Tao and Yin، نويسنده , , Jiao and Deng، نويسنده , , Sisi and Li، نويسنده , , Kebin and Cao، نويسنده , , Yazhong، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
11
From page :
771
To page :
781
Abstract :
Odorant-binding proteins (OBPs) are important parts of insect olfactory systems, and sensitive olfaction is vital for phytophagous insects in host foraging. Electrophysiological studies are helpful in understanding olfactory sensing in Sitobion avenae (Hemiptera: Aphididae), but the functions of odorant-binding proteins in this insect are poorly understood. In this study, we used fluorescence competition assays to measure the binding specificities of SaveOBPs. The results showed that both SaveOBP2 and SaveOBP3 were superior to SaveOBP7 in binding green leaf volatiles. It was unexpected that SaveOBP7 bound trans-β-farnesene strongly, which was known as alarm pheromone of this species. Host volatiles were recognized much more easily by SaveOBP2, and the observed binding activity of SaveOBP2 equaled for tested green leaf volatiles. Our results imply that SaveOBP7 might play a more important role in aphid alarm pheromone discrimination.
Keywords :
odorant-binding proteins , trans-?-farnesene , Sitobion avenae , Fluorescence competition assay , Green leaf volatiles
Journal title :
Journal of Insect Physiology
Serial Year :
2012
Journal title :
Journal of Insect Physiology
Record number :
1416733
Link To Document :
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