Characterization of male-derived factors inhibiting female sexual receptivity in Lygus hesperus

Newly mated females of the plant bug, Lygus hesperus Knight, enter a refractory period during which their sexual receptivity to courting males is greatly reduced for several days. This behavioral change appears to be induced by male-derived factors delivered in the spermatophore during copulation. To better understand the source of the factor(s) responsible for the inhibition, the homogenates of spermatophores, or of the individual organs that provide the constituents of the spermatophore, were injected directly into the abdomen of virgin females. The contents of the lateral and medial accessory glands both appear to produce inhibitory effects, but those of the seminal vesicle had no effect. Treatment of the homogenate also indicated that the active factor(s) is heat labile and water soluble. Several unique proteins were found in the water soluble fraction of the spermatophore, one of which is similar in size to the Drosophila melanogaster sex peptide, a male derived compound known to inhibit receptivity in female flies. In addition, spermatophores contained a substantial quantity of juvenile hormone, a key endocrine regulator of reproductive behavior and physiology in most insects. The results support the hypothesized role of males in manipulating the post-mating behavior of females, and suggest this is achieved through multiple components that act in concert to induce both short- and long-term effects.