Author/Authors :
Mirhendi، H نويسنده Ph.D, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran , , Moazeni، M نويسنده PhD Student of Medical Mycology, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, Iran , , Nikaeen، M نويسنده Assistance professor, Department of Environmental Health Engineering, Isfahan University of Medical Sciences, Isfahan, Iran , , Makimura، K نويسنده Professor, Institute of Medical Mycology and Genome Research Center, Teikyo University, Tokyo, Japan ,
Abstract :
Differentiating and typing clinical and environmental isolates of Aspergillus strains are necessary
for epidemiological studies and could contribute to the solution of several pertinent
clinical problems. We investigated the utility of the RAPD-PCR technique for typing twenty-six
strains of Aspergillus fumigatus and ten additional clinical and environmental Aspergillus
fumigatus and Aspergillus niger isolates. Fungal genomic DNA was extracted using glass bead
disruption and RAPD analysis was performed by using a six individual (R108, R151, UBC90)
and combined (R108+R151, R108+UBS90, R151+UBC90) primer set. Primer pair
R108+UBC90 demonstrated the highest discriminatory power with collection strains, whereas
primer R151 displayed the highest degree of discrimination power with clinical and environmental
isolates. Both primer sets detected seven types of strains.
It was found that primer R151, on its own and in combination with primer UBC90, shared a
relative relationship between clinical and environmental A. fumigatus isolates. We found that
although RAPD-PCR analysis can be applied as a simple, rapid, and very effective method for
differentiating Aspergillus strains, selecting the best primers is a critical step to reach the
desirable discriminatory power.
