Oxygen sensing based on the oxidation process in resorufin dye

By using the planar laser induced florescence experiment (PLIF), a resorufin (RSRF) dye solution was tested as an oxygen probe. The photoreaction of RSRF dye was investigated and the oxidation process of this fluorescent probe molecule was demonstrated. The effect of the oxygen bubbles on tracing dissolved oxygen was also investigated in this study. Results obtained for this sensitive molecular probe dye are very interesting and verify that such an agent can be used effectively for investigating different interesting phenomena affecting the fluorescence. The temporal and spatial behaviors of the oxidation and fluorescence processes were described in this report. For a delay time of 4500 ms the image of the sample cell containing oxygen bubbles was investigated and the distribution of the resulting fluorescence is discussed. For the range of 0.5–2 s, the oxidation and the following fluorescence processes were detectable and the minimum detection time for the given conditions was about 250 ms. The sensitivity of the fluorescent detection was about 140 counts/ms under the given conditions. Considering the response time of the other oxygen probes, it seems that the reported molecular probe can be implemented in design of faster oxygen sensors.