In situ monitoring of nitric oxide release from rat kidney at poly(eosin b)-ionic liquid composite-based electrochemical sensors

A novel amperometric nitric oxide sensor based on poly(eosin b)-ionic liquid composite (PEB-IL) films was described. Conducting PEB was firstly electrodeposited on the surface of a glassy carbon electrode, and then a composite of 1-hexyl-3-methylimidazolium hexafluorophosphate (HIMIMPF6) and didodecyl dimethyl ammonium bromide (DDAB) was coated on the PEB film. The resulting PEB-IL composite has the advantages of fast response and high selectivity for the determination of nitric oxide. Cyclic voltammetry (CV) and amperometric methods were employed to study the electrochemical behavior of the PEB film electrode and its electrochemical response toward NO. The results showed that the PEB-IL composite modified electrode exhibited an excellent anti-interference ability and a wide linear relationship over a NO concentration range of 3.6 × 10−8 to 1.3 × 10−4 mol/L with a low detection limit of 1.8 × 10−8 mol/L (S/N = 3). This sensor was successfully applied to the monitoring of NO release in rat kidney.