Author/Authors :
Fertier، نويسنده , , Laurent and Cretin، نويسنده , , Marc and Rolland، نويسنده , , Marc and Durand، نويسنده , , Jean-Olivier and Raehm، نويسنده , , Laurence and Desmet، نويسنده , , Rémi and Melnyk، نويسنده , , Oleg and Zimmermann، نويسنده , , Céline and Déjous، نويسنده , , Corinne and Rebière، نويسنده , , Dominique، نويسنده ,
Abstract :
A new novel and easy functionalization route of a Love wave acoustic sensor based on the α-oxo-semicarbazone bond is described. The interest is firstly to observe in real-time the immobilization of the peptide on the semicarbazide surface of the transducer and secondly to monitor the specific binding of antibodies. Site-specific immobilization of antigenic-peptides as well as binding of murine monoclonal antibodies has been shown by gravimetric measurements. A tetramethylrhodamine-labeled goat antibody directed against murine antibodies was used to further characterize the biomolecular interactions by fluorescence microscopy and surface analysis (by AFM). Our data show that the gravimetric monitoring developed from the prepared Love wave immunosensor is a promising alternative route to characterize chemical and biomolecular events.
Keywords :
Surface modification , SH-SAW , Real-time antibodies recognition monitoring , Semicarbazide functionalization , SAMs
