Hydrogen peroxide electrochemical detection for the development of protein film-modified sensor

A protein thin film-modified electrode sensor, that features both generalizability and simplicity in design toward reagentless detection of hydrogen peroxide with high sensitivity and reliability, is reported here. Within this electrode device, the film active material of the nanocomposite of myoglobin and zirconium (iv) ion-adenosine monophosphate dianion particles forming via monolayer adsorption of protein, is fabricated on a glassy carbon surface using self-assembly technique. The electrode modification helps in facilitating the direct electron transfer kinetics of protein at the formal potential (E°′) of 12.3 mV versus SHE (pH 7.0). As a result, the potential applied in H2O2 determination through reduction can be shifted to −3.7 mV, a useful characteristic for further applications. Furthermore, the electrode configuration provides sufficient operational stability for sensing. Detection limit of 0.06 μM and the linear calibration range up to 148.47 μM H2O2 are obtained for this sensor. The sensor assay can retain a value of 91.7% initial activity within 1 month.