Author/Authors :
Odorizzi، نويسنده , , L. and Ress، نويسنده , , C. and Collini، نويسنده , , C. and Morganti، نويسنده , , E. and Lorenzelli، نويسنده , , L. and Coppedè، نويسنده , , N. and Alabi، نويسنده , , A.B. and Iannotta، نويسنده , , S. and Cazzanelli، نويسنده , , E. and Vidalino، نويسنده , , L. and Macchi، نويسنده , , P.، نويسنده ,
Abstract :
This work presents an improved platform for single-site electroporation and controlled transfectants delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes for the improvement of cell adhesion. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface using traditional protocols. The system has been previously tested by electroporating HeLa cells with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmid for the enhanced expression of Green Fluorescence Protein (pEGFP-N1) delivered through microchannels.
Keywords :
Cell electroporation , Functionalization , Lab-on-cell , Microelectrode array (MEA) , Microfluidics
