Title of article
Development of a three-dimensional microfluidic system for long-term tumor spheroid culture
Author/Authors
Zi??kowska، نويسنده , , Karina and Kwapiszewski، نويسنده , , Rados?aw and Stelmachowska، نويسنده , , Agnieszka and Chudy، نويسنده , , Michal and Dybko، نويسنده , , Artur and Brzozka، نويسنده , , Zbigniew، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2012
Pages
6
From page
908
To page
913
Abstract
In this paper, we present a low-cost three-dimensional microfluidic system made of poly(dimethylsiloxane) for tumor spheroid formation and cultivation. Tumor spheroids reaching diameters of up to 300 μm were cultured in perfusion microchambers providing extremely low shear stress. Spheroids were cultured for 15 days and high viability of the cells was observed within this time. The presented microsystem is a prospective solution for development of microfluidic platforms for anticancer drug screening.
Keywords
lab-on-a-chip , Tumor spheroid , Long-term cell culture , Three-dimensional microfluidic system
Journal title
Sensors and Actuators B: Chemical
Serial Year
2012
Journal title
Sensors and Actuators B: Chemical
Record number
1441089
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