Title of article :
Characterization of an ultrasensitive biosensor based on a nano-Au/DNA/nano-Au/poly(SFR) composite and its application in the simultaneous determination of dopamine, uric acid, guanine, and adenine
Author/Authors :
Niu، نويسنده , , Ling Mei and Lian، نويسنده , , Kao Qi and Shi، نويسنده , , Hong Mei and Wu، نويسنده , , Yi Bing and Kang، نويسنده , , Wei Jun and Bi، نويسنده , , Si Yuan، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Abstract :
An ultrasensitive method for the simultaneous determination of dopamine (DA), uric acid (UA), guanine (G), and adenine (A) was developed using differential pulse voltammetry with a three-dimensionally distributed Au nanoparticle (GNP)-modified glassy carbon electrode (GCE). The nano-Au/DNA/nano-Au/poly(SFR)/GCE microstructure was characterized by scanning electron microscopy, electrochemical impedance spectroscopy, and atomic force microscopy, showing an anchored three-dimensional distribution of GNPs on the modified electrode. The electrode exhibited ultrasensitive responses to DA, UA, G, and A due to poly(SFR) electrocatalytic activities and the large surface area of the GNPs. All four analytes showed well-defined catalytic oxidation peaks at the modified electrode. DA, UA, G, and A yielded linear ranges from 8.0 × 10−9 M to 1.1 × 10−6 M, 9.0 × 10−8 M to 1.2 × 10−5 M, 9.0 × 10−9 M to 5.0 × 10−6 M, and 6.0 × 10−8 M to 8.0 × 10−7 M, respectively, and detection limits for the analytes were 2.0 × 10−10 M, 8.0 × 10−9 M, 5.0 × 10−10 M, and 4.0 × 10−9 M, respectively. The feasibility of the proposed assay for use in human serum and urine was investigated and satisfying results were obtained.
Keywords :
Gold nanoparticles , Dopamine , guanine , uric acid , adenine
Journal title :
Sensors and Actuators B: Chemical
Journal title :
Sensors and Actuators B: Chemical