Author/Authors :
Kurunczi، نويسنده , , S. and Hainard، نويسنده , , A. L. Juhasz، نويسنده , , K. and Patko، نويسنده , , D. and Orgovan، نويسنده , , N. and Turck، نويسنده , , N. and Sanchez، نويسنده , , J.C. and Horvath، نويسنده , , R.، نويسنده ,
Abstract :
Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor.
Keywords :
Polyethylene imine (PEI) , Room temperature immobilization , Neutravidin , C-reactive protein (CRP) , owls , Label free sensor
