Bienzyme colorimetric detection of glucose with self-calibration based on tree-shaped paper strip

In this contribution, we first developed a semiquantitative method for the detection of glucose with self-calibration based on bienzyme colorimetry by using tree-shaped paper strip. The GOD/HRP bienzyme system was utilized to amplify the color signal in the aqueous phase. Moreover, we employed a paper as microfluidic media for running colorimetric assay, while tree-shaped paper strip was designed to ensure uniform microfluidic flow for multiple branches. Our proposed method gives direct outcomes which can be observed by the naked eye or recorded by a simple camera. The linear range is from 1.0 × 10−3 to 11.0 × 10−3 M, with a detection limit of 3 × 10−4 M. Furthermore, the effect of detection condition has been investigated and discussed comprehensively. The result of determining glucose in human serum is consistent with that of detecting standard glucose solution by using our developed approach. A low-cost, simple, and rapid colorimetric method for the simultaneous detection of glucose with self-calibration on the tree-shaped paper has been proposed.