Author/Authors :
Bohrn، نويسنده , , Ulrich and Mucha، نويسنده , , Andreas and Werner، نويسنده , , Carl Frederik and Trattner، نويسنده , , Barbara and Bنcker، نويسنده , , Matthias and Krumbe، نويسنده , , Christoph and Schienle، نويسنده , , Meinrad and Stütz، نويسنده , , Evamaria and Schmitt-Landsiedel، نويسنده , , Doris and Fleischer، نويسنده , , Maximilian and Wagner، نويسنده , , Patrick and Schِning، نويسنده ,
Abstract :
The toxicity of chromium ions was investigated using mammalian cell cultures on impedance sensors as well as physiological in vitro sensor systems. The performance of commercially available systems like the 2500 Analyzing System (Bionas), xCELLigence (Roche) and Cytosensor Microphysiometer (Molecular Devices) was compared with a novel CMOS-based impedance-to-frequency converter device. Cell-based sensor systems are shown to be powerful tools to detect Cr(VI) pollutions within several hours in the range of multinational drinking water regulations. The ability to distinguish between toxic Cr(VI) and non-toxic Cr(III) species is one advantage of these integral sensor systems. Impedance only devices are not sufficient for the fast detection of toxic chromium species as rapid cellular changes occur only in the respiration system and the cell physiology.
Keywords :
Cell-based biosensor , Chromium , Impedance , acidification , respiration , V79
