Title of article
A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging
Author/Authors
Bell، نويسنده , , Laurence and Seshia، نويسنده , , Ashwin and Lando، نويسنده , , David and Laue، نويسنده , , Ernest and Palayret، نويسنده , , Matthieu and Lee، نويسنده , , Steven F. and Klenerman، نويسنده , , David، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2014
Pages
6
From page
36
To page
41
Abstract
We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
Keywords
single cell analysis , Microfluidics , Hydrodynamic trapping , PALM imaging , Single molecule localisation microscopy
Journal title
Sensors and Actuators B: Chemical
Serial Year
2014
Journal title
Sensors and Actuators B: Chemical
Record number
1442291
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