Development of oligopeptide-based novel biosensor by solid-phase peptide synthesis on microchip

A miniaturized solid-phase peptide synthesis (SPPS) system was developed aiming a novel concept of programmable biosensor that can flexibly change the target on-site and on-demand. Oligopeptides, utilized as probes for the biosensor, were synthesized by the on-chip SPPS on a single bead trapped at the center of a micro channel. After the completion of probe synthesis, a sample solution containing target was exposed to the beads. The selective bindings of targets and the synthesized oligopeptide probes were optically measured. As examples of sensing, YOYO1 label DNA binding with three probe sequences RHKS, EDED (hydrophilic), LAGV (hydrophobic), and also Ni binding with His-tag were investigated. Sequence-dependent different affinity and selectively bindings of these targets were confirmed. These results demonstrated the concept of a programmable biosensor.