Triple catalysis amplification strategy for simultaneous multiplexed electrochemical immunoassays based on cactus-like MnO2 functionalized nanoporous gold

A novel triple catalysis amplification strategy for simultaneous multiplexed electrochemical immunoassays based on cactus-like MnO2 functionalized nanoporous gold (NPG-MnO2) was designed. The NPG-MnO2 with enhanced catalytic activity, electrical conductivity and solubility was synthesized by an efficient, green and controllable approach. The obtained material was further characterized and used as matrices to immobilize the redox probes to produce a multiplexed electrochemical immunoassay. With the employment of the as prepared bioconjugates, triple catalysis amplification strategy was described duo to the enhanced catalytic effects of horseradish peroxidase (HRP) and two bio-mimetic enzymes (NPG and MnO2) through sandwich-type immunoassay protocol.