Title of article :
Lipid Peroxidation in Sarcoplasmic Reticulum Membranes: Effect on Functional and Biophysical Properties
Author/Authors :
Dinis، نويسنده , , T.C.P. and Almeida، نويسنده , , L.M. and Madeira، نويسنده , , V.M.C.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1993
Pages :
9
From page :
256
To page :
264
Abstract :
The fluorescent polyunsaturated parinaric acid (PnA) incorporated in sarcoplasmic reticulum membranes (SR) was used to probe the initial stages of membrane lipid peroxidation. The experimental set up of the PnA assay was investigated by means of several peroxidation initiators to ascertain peroxidation conditions. This assay in SR is particularly useful to evaluate the membrane susceptibility to peroxidation and to ascertain suitable conditions (concentration of initiators and cofactors) to challenge peroxidation in each preparation under study. On the basis of the PnA assay, Fe2+ /ascorbate was selected among the different initiator systems to assess the effect of lipid peroxidation upon biochemical and biophysical parameters of SR membranes. Under mildly controlled conditions at 25°C, the lipid degradative process, as detected by fatty acid analysis, decreases the Ca2+ uptake (up to about 50% of control) and reduces the Ca2+ pump efficiency (Ca2+ /ATP ratio) up to about 58% of control, without inactivation the ATPase enzyme turnover. The effect of lipid peroxidation on the SR bilayer organization is dependent either on the extent of lipid peroxidation or on the depth of the bilayer as probed by fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene and by intramolecular excimerization of 1,3-di(1 -pyrenyl)propane. It is concluded that the effect of mild lipid peroxidation on Ca2+ pump activity is partially exerted through the alteration of physical properties in the lipid phase or lipid-protein interfaces.
Journal title :
Archives of Biochemistry and Biophysics
Serial Year :
1993
Journal title :
Archives of Biochemistry and Biophysics
Record number :
1450131
Link To Document :
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