Porcine troponin I: a thermostable species marker protein

In this study, we confirmed our previous hypothesis that the 24 kD thermostable skeletal muscle protein (TSMP) recognized by a panel of porcine-specific monoclonal antibodies (MAbs) is skeletal troponin I (sTnI). The TSMP and sTnI purified from porcine muscle have identical electrophoretic mobilities, isoelectric characteristics, and specific antigenicities. The heterogeneity of sTnI between porcine and other species, and between porcine sTnI and other troponin subunits or cardiac isoforms can be immunologically differentiated by the MAbs. Heat treatment of sTnI up to 126 °C for 120 min did not diminish its solubility and antigenicity. The antigenic specificity and thermal stability of sTnI indicate its potential as a thermostable species marker for the identification of the origin of meats in severely heated products.