Title of article
Reversible immobilization of engineered molecules by Ni-NTA chelators
Author/Authors
Maly، نويسنده , , J and Di Meo، نويسنده , , C and De Francesco، نويسنده , , M and Masci، نويسنده , , A and Masojidek، نويسنده , , J and Sugiura، نويسنده , , M and Volpe، نويسنده , , A and Pilloton، نويسنده , , R، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
5
From page
271
To page
275
Abstract
Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)6-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1–12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor μ-array.
Keywords
Biosensors , Photosystem II , engineered proteins , Reversible immobilization , Self-assembled monolayers , Conducting molecular wires , (His)6-tag
Journal title
Bioelectrochemistry
Serial Year
2004
Journal title
Bioelectrochemistry
Record number
1451086
Link To Document