Title of article
Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly
Author/Authors
Calvo-Muٌoz، نويسنده , , M.-L. and Dupont-Filliard، نويسنده , , A. and Billon، نويسنده , , M. and Guillerez، نويسنده , , S. and Bidan، نويسنده , , G. and Marquette، نويسنده , , C. and Blum، نويسنده , , L.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
5
From page
139
To page
143
Abstract
The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
Keywords
ECL , Polypyrrole , DNA , Sensor , detection , ABEI
Journal title
Bioelectrochemistry
Serial Year
2005
Journal title
Bioelectrochemistry
Record number
1451387
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