Title of article
Real-time assay of immobilized tannase with a stopped-flow conductometric device
Author/Authors
Chang، نويسنده , , Fu-Shiang and Chen، نويسنده , , Po-Chung and Chen، نويسنده , , Richie L.C. and Lu، نويسنده , , Fu-Ming and Cheng، نويسنده , , Tzong-Jih، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
4
From page
113
To page
116
Abstract
A stopped-flow manifold was developed to assay and characterize immobilized tannase (EC 3.1.1.20). The immobilized enzyme reactor was inserted within the tube-type electrode pair (cell constant = 103.2 cm− 1) for a real-time conductometric measurement. Tris buffer (2 mM, pH = 7.0) was used as the carrier for sensitivity improvement. The activities and kinetic parameters (Km values) for propyl gallate, methyl gallate and tannic acid were investigated.
Keywords
Conductometric , enzyme kinetics , Tannic acid , stopped-flow
Journal title
Bioelectrochemistry
Serial Year
2006
Journal title
Bioelectrochemistry
Record number
1451531
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