Prediction of pH-Dependent Properties of DNA Triple Helices

The thermodynamic properties of two triple helices were investigated by uv thermal denaturation, differential scanning calorimetry, and pH titrations. Starting from the grand partition function and using matrix methods we present a formalism that describes pH effects on the thermal stability of triple helices. The formalism can be used over a wide pH range and is not restricted to the limiting case where the pH is larger or smaller than the pKa of cytosine. Furthermore, it covers nearest neighbor electrostatic effects of closely spaced cytosines in the Hoogsteen strand which can shift the pKa of cytosine to lower pH values. A procedure is employed to predict enthalpy and entropy changes for tripler formation. These values are in accordance with the results obtained by differential scanning calorimetry.