Author/Authors :
B??lkov?، نويسنده , , Zuzana and Slov?kov?، نويسنده , , Marcela and Ly?ka، نويسنده , , Anton??n and Hor?k، نويسنده , , Daniel and Lenfeld، نويسنده , , Ji??? and Turkov?، نويسنده , , Jaroslava and Chur??ek، نويسنده , , Jaroslav، نويسنده ,
Abstract :
In order to obtain an active and stable oxidation reactor for daily use in biochemical laboratory we decided to immobilize galactose oxidase orientedly through a carbohydrate chain to the magnetic carriers. We used hydrazide derivatives of non-magnetic and magnetic bead cellulose and of magnetic and non-magnetic poly(HEMA-co-EDMA) microspheres. Activation of the enzyme molecules was done by sodium periodate in the presence of supplements (fucose, CuSO4, catalase). Orientedly immobilized galactose oxidase presents high storage stability and lower susceptibility to inappropriate microenvironmental conditions. Reactor reactivated by three pulses of d-galactose retained practically 100% of its native activity after 6 months. The positive properties of both magnetic carriers were entirely confirmed.
