Simple and sensitive method for the determination of chlorpheniramine maleate in human plasma using liquid chromatography–mass spectrometry

A convenient liquid chromatographic–single quadrupole mass spectrometric (LC–MS) method was developed and validated for the determination of chlorpheniramine maleate (INN name: chlorphenamine) in human plasma. The method had advantages of a single liquid–liquid extraction with diethylether and high sensitivity. The linearity was also excellent over the concentration range of 0.52–20.8 ng/ml of chlorpheniramine maleate. The intra- and inter-day precision and accuracy ranged between 0.0 and 13.9%, showing a good reproducibility. This developed method was successfully applied to analysis of chlorpheniramine maleate in clinical studies.