Author/Authors :
Mühling، نويسنده , , Jِrg and Fuchs، نويسنده , , Markus and Campos، نويسنده , , Marie E and Gonter، نويسنده , , Jens and Engel، نويسنده , , Jِrg M and Sablotzki، نويسنده , , Armin and Menges، نويسنده , , Thilo and Weiss، نويسنده , , Stefan and Dehne، نويسنده , , Marius G and Krüll، نويسنده , , Matthias and Hempelmann، نويسنده , , Gunter، نويسنده ,
Abstract :
For the first time, a procedure is described for the quantitative analysis of free α-keto acid content in human neutrophils (PMNs) relative to single cell number by reversed-phase fluorescence high-performance liquid chromatography. The procedure is minimally invasive and is unsurpassed in the quality of PMN separation, ease of sample preparation as well as sample stability. This method can satisfy the rigorous demands for an ultra-sensitive, comprehensive and rapid intracellular α-keto acid analysis in particularly for the surveillance of severe diseases as well as cellular or organ dysfunction.
