Author/Authors :
Sato، نويسنده , , Kenji and Tanahashi-Shiina، نويسنده , , Tamae and Jun، نويسنده , , Feng and Watanabe-Kawamura، نويسنده , , Atsuko and Ichinomiya، نويسنده , , Masami and Minegishi، نويسنده , , Yutaka and Tsukamasa، نويسنده , , Yasuyuki and Nakamura، نويسنده , , Yasushi and Kawabata، نويسنده , , Makoto and Ohtsuki، نويسنده , , Kozo، نويسنده ,
Abstract :
A chromatographic method is described for purification of type V collagen, a minor constituent in extracellular matrix, from a pepsin digest of porcine intestinal connective tissue. The starting material was a viscous and turbid solution even after centrifugation. Direct application of the sample to a commercially available DEAE-cellulose column resulted in clogging. On the other hand, type V collagen, [α1(V)]2α2(V) form, was successfully captured by a filter paper-based DEAE-cellulose column chromatography and purified by a subsequent commercially available cation-exchange medium without clogging. This is a vast improvement over previously described salt fractionation methods.
