Development and validation of a liquid chromatographic method for Casiopeina IIgly® in rat plasma

A sensitive and specific liquid chromatographic method using solid-phase extraction with Sep-pak cartridges has been developed for the determination of Casiopeina IIgly and validated over the linear range 2.5–50 μg/ml in rat plasma. The analysis was performed on a Symetry C18 (5 μm) column with a Phenomenex C18 precolumn. The mobile phase was methanol–water (58:42, v/v). The column effluent was monitored at 273 nm. The results showed that the assay is sensitive at 2.5 μg/ml. Maximum intra-day coefficient of variation was 11.47%. The recovery based upon addition of internal standard to rat plasma was 80.98%. The method was used to perform preclinical pharmacokinetic studies in rat plasma and was found to be satisfactory.