Simultaneous determination of bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography–mass spectrometry

We devised a sensitive and simple method to simultaneously determine bromvalerylurea and allylisopropylacetylurea in human blood and urine by gas chromatography–mass spectrometry. Bromvalerylurea and allylisopropylacetylurea were extracted using an Extrelut® column with an internal standard, 2-bromohexanoylurea, followed by derivatization with heptafluorobutyric anhydride. The derivatized extract was submitted to GC–MS analysis of EI-SIM mode. The calibration curves of both compounds were linear in the concentration range from 0.01 to 10 μg/ml in both blood and urine samples. The lower limits of detection of bromvalerylurea and allylisopropylacetylurea were 0.005 and 0.005 μg/ml, respectively. This method proved most useful in accurately identifying these drugs in blood and urine from an autopsied individual.