Author/Authors :
Zuppi، نويسنده , , Cecilia and Rossetti، نويسنده , , Diana Valeria and Vitali، نويسنده , , Alberto and Vincenzoni، نويسنده , , Federica and Giardina، نويسنده , , Bruno and Castagnola، نويسنده , , Massimo and Messana، نويسنده , , Irene، نويسنده ,
Abstract :
We propose a method for the simultaneous determination of hippuric acid (HA) and creatinine based on capillary micellar electrokinetic chromatography. Experimental conditions were 20 mM sodium phosphate, pH 7.20, 25 mM sodium dodecyl sulfate, 5% (v/v) acetonitrile. Electropherograms evidenced HA and creatinine peaks in less than 12 min. The method showed good linearity for both analytes and satisfactory within-day precision. The present method, which is accurate, sensitive, rapid and simple, may be applied to single-spot urine samples.