Title of article
Application of liquid chromatography–electrospray ionization mass spectrometry for study of steroid-converting enzymes
Author/Authors
Mik??́k، نويسنده , , Ivan and Mikul?́kov?، نويسنده , , Kate?ina and P?cha، نويسنده , , Ji??́ and Ku?ka، نويسنده , , Marek and Deyl، نويسنده , , Zden?k، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
9
From page
145
To page
153
Abstract
A high-performance liquid chromatography–atmospheric pressure ionization–electrospray ionization mass spectrometry (HPLC–API–ESI–MS) method was developed for the analysis of steroids in a study of steroid-converting enzymes. Separations ware done on a Zorbax Eclipse XDB-C18 column (eluted with a linear methanol–water–acetic acid gradient) and identification of the steroids involved was done by API–ESI–MS using positive ion mode and extracted ion analysis. The applicability of the present method for studying steroid metabolism was proven in assaying two steroid-converting enzymes (20β-hydroxysteroid dehydrogenase and 11β-hydroxysteroid dehydrogenase) in various biological samples (rat and chicken intestine, chicken oviduct).
Keywords
enzymes , Steroids , steroid-converting
Journal title
Journal of Chromatography B
Serial Year
2004
Journal title
Journal of Chromatography B
Record number
1456417
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