Author/Authors :
Loret، نويسنده , , S. and Nollevaux، نويسنده , , G. and Remacle، نويسنده , , R. and Klimek، نويسنده , , M. and Barakat، نويسنده , , I. and Deloyer، نويسنده , , P. and Grandfils، نويسنده , , C. and Dandrifosse، نويسنده , , G.، نويسنده ,
Abstract :
We developed a treatment of urine samples allowing the analysis of two intestinal permeability markers: polyethylene glycol (PEG) 400 (highly diffusible; basal permeability indicator) and PEG 4000 (poorly diffusible; indicator of an abnormal increase of permeability) by a unique gel permeation chromatography (GPC) with refractometric detection. Urinary PEG were extracted using a mixed-bed resin composed of C2 and C18 layers. Permeability mean values determined in 11 human healthy subjects were 24.20±9.30% and 0.12±0.08% for, respectively, PEG 400 and 4000. The percentage of the PEG 4000 permeability value to the one of PEG 400 corresponded to an intestinal permeability index (IPI) of 0.52±0.35 expressing a low diffusion of this poorly permeability marker.
