Author/Authors :
Horneman، نويسنده , , D.A and Wolbers، نويسنده , , M. Zomerdijk، نويسنده , , M and Ottens، نويسنده , , M and Keurentjes، نويسنده , , J.T.F and van der Wielen، نويسنده , , L.A.M، نويسنده ,
Abstract :
The flexibility and selectivity of size exclusion chromatography (SEC) for protein purification can be modified by adding non-ionic micelle-forming surfactants to the mobile phase. The micelles exclude proteins from a liquid phase similar to the exclusion effect of the polymer fibers of the size exclusion resin. This surfactant-aided size exclusion chromatography technology (SASEC) is demonstrated on the separation of two model proteins; bovine serum albumin (BSA) and myoglobin (Myo). The effect of the added surfactants on the distribution behavior of the proteins is predicted adequately by a size exclusion model presented in this paper.
