Title of article :
Effect of electrostatic energy on partitioning of proteins in aqueous two-phase systems
Author/Authors :
Olivera-Nappa، نويسنده , , A. and Lagomarsino، نويسنده , , G. and Andrews، نويسنده , , B.A. and Asenjo، نويسنده , , J.A.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
6
From page :
81
To page :
86
Abstract :
An attempt has been made to adopt a different approach to evaluate the effect of a protein’s charge on its partitioning behaviour in PEG/salt aqueous two-phase systems (ATPS). This has been done using a computer methodology (DelPhi) that allows the calculation of the electrostatic solvation energy that charged proteins present in a particular media such as aqueous polymer–salt systems. This calculation was done for the protein in each of the phases and a correlation was investigated that related the electrostatic energy difference of the protein in each of the phases and its partition coefficient in ATPS. Such correlation resulted in a statistical model that also included the effect of molecular weight and a shape factor at each particular pH. A global correlation which included the effect of pH was also found. All the correlations were statistically evaluated and gave good results.
Keywords :
Proteins , Aqueous two-phase systems , Partitioning , electrostatic energy
Journal title :
Journal of Chromatography B
Serial Year :
2004
Journal title :
Journal of Chromatography B
Record number :
1456825
Link To Document :
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