Title of article
Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma
Author/Authors
B. Bindukumar، نويسنده , , B. and Kawinski، نويسنده , , Elzbieta and Cherrin، نويسنده , , Craig and Gambino، نويسنده , , Leah M. and Nair، نويسنده , , Madhavan P.N. and Schwartz، نويسنده , , Stanley A. and Chadha، نويسنده , , Kailash C.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
8
From page
113
To page
120
Abstract
The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.
Keywords
prostate cancer , prostate-specific antigen , PSA , PSA purification , Thiophilic-interaction chromatography , serine protease
Journal title
Journal of Chromatography B
Serial Year
2004
Journal title
Journal of Chromatography B
Record number
1457078
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