Hydrogen Peroxide Induces the Expression ofadapt15,a Novel RNA Associated with Polysomes in Hamster HA-1 Cells

In mammalian cells, hydrogen peroxide is known to induce the expression of several genes thought to be important in protection against oxidative stress. Using mRNA differential display, we have identified a novel RNA in hamster HA1 cells that is strongly induced by hydrogen peroxide under conditions where a protective adaptive response occurs. This induction was observed between 1 and 18 h after peroxide treatment, and at an H2O2concentration that caused little or no cytotoxicity. Northern blot analysis revealed that this major inducible RNA species, termedadapt15,is 950 bases in size. Two versions of this RNA were found by sequence analysis, differing only by a short trinucleotide stretch. Despite polyadenylation, no large open reading frame was observed. Fractionation studies, however, indicate thatadapt15RNA is primarily located in the cytoplasm, and a significant percentage of it is associated with active translation.adapt15RNA may act at the level of translation to protect cells against the damaging effects of oxidative stress.