Author/Authors :
Meraj، Munazzah نويسنده Department of Biochemistry, Peoples University of Medical & Health Sciences Nawabshah Pakistan , , Rahman، Khalilur نويسنده Lecturer (Accounting), National University, Gazipur-1704, Bangladesh , , Javed، Sadia نويسنده Department of Chemistry and Biochemistry, GC University , , Irfan، Rao نويسنده Department of Pharmaceutics, University of Sindh, Jamshoroo ,
Abstract :
This research work was focused on the hyperproduction of urate oxidase through mutagenesis of Bacillus subtilis. The strain was subjected to chemical mutagenesis. Ethyl methane sulfonate treated Bacillus subtilis (BEM-2) at 180 minutes dose rate was showed the best for optimum yield of urate oxidase. For obtaining optimum yield of enzyme the fermentation medium of parental and mutated strains was optimized individually. It was observed that 0.5% substrate (uric acid) concentration, 36 hours fermentation period, 8.5 pH, 35 oC temperature, 0.3% yeast extract and 2% sucrose, enhanced the activity of the parent (19.48±1.26 U/mg) and mutant (68.05±3.50 U/mg) derived enzyme. The nutritional requirements of mutant Bacillus subtilis observed to be the same as those of wild/parent strain.
